Edit for question clarification: It is known that Darwin came into contact with la vinchuca (Triatoma infestans, the vector for Trypanosoma cruzi) during his voyage on the beagle. There exists an account of his group observing la vinchuca biting the skin of humans. Besides contact with the vector, what other evidence suggests he developed the disease?
Can you give some detail as to why you think he might have had Chagas Disease?