I think one could get into a lot of pedantry about what it means to be "non-test" (e.g., there were uses of nuclear weapons that were not "tests" per se — such as when the Soviets detonated some underground to shut off a natural gas fire), but if you are asking whether any have been used "in anger," in the sense of one country trying to kill others with them directly, the answer is no. Nagasaki was the "last bomb" in that sense. So far.