I'm referring to the incident during which APPARENTLY, due to a letter that nobunaga's daughter who was married to ieyasu's son, sent to Nobunaga, trying to frame her mother in law, ended up causing Nobunaga to have suspicions towards the Tokugawa, and things so bad that Ieyasu had to execute his wife and son to ease the tension.
Do we know, if he still resented Nobunaga afterwards ? I would hope so, but in that era you never know.
PS: I may gotten some details wrong as this affair isn't quite clear to me
For the current scholar consensus on what actually happened, and who's to be blamed, in the Nobuyasu Incident, see here.